Lectures
1. Advanced Metabolic Engineering
“Advanced Metabolic Engineering aims the purposed redesign of the biological systems in the level of metabolism and covers the basic review of metabolism, various experimental methods to understand metabolic pathways, Metabolic Control Analysis (MCA), Metabolic Flux Analysis (MFA), Flux Balance Analysis (FBA), and genome-scale metabolic analysis. In addition, its applications to Industrial, Medical, and Agricultural Biotechnology are illustrated.
Pre-requisite course
None
Evaluation
Mid-term 30 %, Final 40 %, Homework 20%, Attendance 10 %
Testbook
Metablic Engineering” G.N. Stephanopoulos, A.A. Aristidou, J. Nielsen (1998) Academic Press
Reference
“Systems Biology: Properties of reconstructed networks” B.O. Palsson (2006) Cambridge University Press
Plan
Week 1: Introduction
Week 2 – 3 : Reviews on Metabolism
Week 4 – 7 : Experimental Methods for Metabolic Analysis
Week 8 – 10 : Metabolic Control Analysis
Week 11 – 13 : Metabolic Flux Analysis
Week 14 – 15 : Metabolic Balance Analysis & Genome-scale metabolic analysis
Week 16 : Applications of Metabolic Engineering
2. Transcription Regulation for Synthetic Biotechnology
This graduate-level course aims to provide intensive knowledge of transcription mechanism and regulation system for synthetic biology especially for the purposeful redesign of the biological system.
Pre-requisite course
None
Evaluation
Mid-term: 30 %, Final: 40 % Homework: 20 %, Attendance: 10 % (including Quiz)
Testbook
R. Wagner : Transcription Regulation in Prokaryotes, Oxford Press (2000)
Reference
1. Biochemistry, 5th ed. L. Stryer, W.H. Freeman & Co. 2007
2. Lehninger Principles of Biochemistry, D.L. Nelson M.M. Cox, Worth Publisher, 2000
3. Molecular Biology of the Cell, B. Alberts et al., Garland Science, 2008
4. Genes VIII B. Lewin, Pearson Prentice Hall, 2004
Plan
Week 1 : Introduction
Week 2 – 3 : The players or cellular components necessary for transcription
Week 4 – 5 : Initiation of transcription
Week 6 – 7 : Transcription elongation
Week 8 : Mid-term exam
Week 9 – 10 : Termination of transcription
Week 11 – 12 : The role of DNA structure in transcription regulation
Week 13 – 14 : Regulation by transcription factors
Week 15 : Regulatory networks
Week 16 : Final exam
3. Protein Biosynthesis
This is an intensive course to study protein synthesis mechanism as well as regulation network in the biological system.
Pre-requisite course
None
Evaluation
Final Exam: 60 %, Review & Presentation: 40 %
Testbook
1. K.H. Nierhaus and D.N. Wilson Protein Synthesis and Ribosome Structure, Wiley-VCH (2004)
Reference
1. Biochemistry 5th ed. L. Stryer, W.H. Freeman & Co.
2. Genes VIII B. Lewin, Prentice Hall (2003)
Plan
Week 1 : Introduction
Week 2 : Initiation of protein synthesis (I)
Week 3 : Initiation of protein synthesis (II)
Week 4 : The elongation cycle (I)
Week 5 : The elongation cycle (II)
Week 6 : Termination of ribosome recycling (I)
Week 7 : Termination of ribosome recycling (II)
Week 8 : mRNA decay and RNA-degrading machines in prokaryotes and eukaryotes (I)
Week 9 : mRNA decay and RNA-degrading machines in prokaryotes and eukaryotes (II)
Week 10 : The mechanism of recoding in pro- and eukaryotes
Week 11 : Antibiotics and the inhibition of ribosome function
Week 12 : The work of chaperones
Week 13 : Break (Self-study)
Week 14 : Final exam
Week 15 : Review Presentation
Week 16 : Review Presentation